| Name of Transformer | Vehicle Mode(s) | Transformer Personality |
| Optimus Prime | Peterbilt 379/389, Western Star 5700 Phantom, 973 Marmon 97, Modern CEO Truck Cab, Dodge Ram SRT-10, A fire truck, Freightliner FL-86 | Optimus Prime is as true and noble being the universe has seen. It is a firm belief that freedom is the right for all beings, and there is good in everyone. |
| Bumblebee | 1977 2nd-gen. Chevy Camaro, 2006 5th-gen. Chevy Camaro, 2010/2011 Chevy Camaro ZL1 6LE, 1967 Chevy Camaro SS, 2014 Chevy Camaro Concept, Mercedes-Benz 770, 2016 Chevy Camaro | Usually presented as the kid of the transformers, Bumblebee is the youngest Autobot and therefore he is brave, friendly and always wanting to show courage to his elder friends. |
| Grimlock | Most commonly as a robotTyrannosaurus Rex | Often aggressive, brutish, stubborn, and short-tempered. He hates the weak and only respects the strong. Since he sees himself stronger than anyone else he is quite arrogant. |
| Arcee | Honda S2000, 2006 Buell Firebolt XB12R Motorcycle/Speedbike, Ducati 848 Superbike | An excellent warrior, shes a melee expert and will not hesitate to take on enemies herself. |
| Ratchet | Hammer H2 Search & Rescue SUV, most commonly as an ambulance, Nissan C2, SUV | Ratchet is always portrayed as a Autobot Physician and combat medic. |
| Ultra Magnus | Car Carrier | Firm, stern, but a fair leader. Prefers to follow the exact protocol. Required or expect Autobots to call him “Sir.” |
| Hot Rod/Rodimus Prime | Cybertronian racer, Space Winnebago, Ford GT | As Hot Rod, he is often portrayed as energetic, yet brash and headstrong, with an overwhelming self-confidence that borders arrogance. As Rodimus Prime, he is more mature, physically more powerful and instilled with the wisdom of the previous leaders who held the Matrix or leadership. |